By William S. M. Wold, Ann E. Tollefson
Adenovirus equipment and Protocols, moment variation, now in volumes, is a vital source for adenovirus (Ad) researchers starting within the box, and an inspirational place to begin for researchers trying to department into new parts of advert learn. as well as updating and increasing the 1st variation, the authors have further new chapters that handle cutting edge components of emphasis in advert learn, together with advert vector development and use, real-time PCR, use of recent animal versions, and strategies for quantification of advert virus or virus expression/interactions. all the protocols offered in those volumes is written through trendsetting researchers.
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Additional resources for Adenovirus Methods and Protocols: Adenoviruses, Ad Vectors, Quantitation, and Animal Models
027 g of Na2HPO4 in a total volume of 90 mL of distilled H2O. 96 with HCl at room temperature, and adjust the volume to 100 mL with distilled H2O. Filter-sterilize and store in aliquots at –20°C. 8. 8 g Na2HPO4 to a final volume of 1 L in water. Autoclave and store at room temperature. 9. 4. Store at 4°C and filter-sterilize aliquots before use. 10. 19 g of HEPES in a total volume of 90 mL distilled H2O. 4 and the volume to 100 mL with distilled H2O. Store at 4°C. 11. 16 g CsCl in 100 mL HEPES-buffered saline (modified).
Immunol. 272, 287–330. 3. , Kimberly, W. , Levine, A. , and Shenk, T. (1997) Nuclear export of the E1B 55-kDa and E4 34-kDa adenoviral oncoproteins mediated by a rev-like signal sequence. EMBO J. 16, 4276–4284. Early Region 1 and 4 Manipulation 39 4. , et al. (2000) The adenovirus type 5 E1B-55k oncoprotein is a highly active shuttle protein and shuttling is independent of E4orf6, p53 and Mdm2. Oncogene 19, 850–857. 5. Weigel, S. and Dobbelstein, M. (2000) The nuclear export signal within the E4orf6 protein of adenovirus type 5 supports virus replication and cytoplasmic accumulation of viral mRNA.
And Gonzalez, R. A. (2003) Regulation of mRNA production by the adenoviral E1B 55-kDa and E4 Orf6 proteins. Curr. Top. Microbiol. Immunol. 272, 287–330. 3. , Kimberly, W. , Levine, A. , and Shenk, T. (1997) Nuclear export of the E1B 55-kDa and E4 34-kDa adenoviral oncoproteins mediated by a rev-like signal sequence. EMBO J. 16, 4276–4284. Early Region 1 and 4 Manipulation 39 4. , et al. (2000) The adenovirus type 5 E1B-55k oncoprotein is a highly active shuttle protein and shuttling is independent of E4orf6, p53 and Mdm2.
Adenovirus Methods and Protocols: Adenoviruses, Ad Vectors, Quantitation, and Animal Models by William S. M. Wold, Ann E. Tollefson